猪繁殖与呼吸综合征病毒感染猪肺泡巨噬细胞天然免疫信号通路相关分子变化分析

doi:10.11843/j.issn.0366-6964.2017.07.013

Abstract

Abstract:

This research was conducted to analyze the proteomic dynamic proteins of PRRSV-infected PAMs. In this study, Label-free LC-MS/MS was employed to quantitatively identify the differentially expressed proteins between PRRSV-infected PAMs and uninfected PAMs. Results were as follows:430 significantly altered proteins were identified after PRRSV infection. Among them, 171 proteins were up-regulated, and 259 proteins were down-regulated. These differentially expressed proteins are related to innate immunity, virus response and antigen presentation etc. Interestingly, many upregulated proteins were enriched in NF-κB, MAPK and RIG-1 signal pathway, including STAT1/2, OAS1/2, Mx1/2, ISG15/20, IFIT1/2/3/5; some proteins were enriched in MHCⅠand MHCⅡrelated signal pathways. Real-time quantitative reverse transcription polymerase chain reaction and Western blot analysis verifiedSTAT 1/2, OAS 1/2, CD 14, ISG, Mx1, IFIT genes and Mx1, IFIT3 proteins were significant up-regulated after PRRSV infection which were consistent with LC-MS/MS results. This study may accelerate our understanding of the mechanisms of PRRSV infection.

CLC Number:

S852.659.6

LI Hua-wei, CHEN Xin-xin, ZHAO Meng-meng, ZHOU En-min, QIAO Song-lin, ZHANG Gai-ping. Analysis of Related Molecular Changes of Innate Immune Signaling Pathway in Porcine Reproductive and Respiratory Syndrome Virus Infected PAMs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1288-1299.

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Analysis of Related Molecular Changes of Innate Immune Signaling Pathway in Porcine Reproductive and Respiratory Syndrome Virus Infected PAMs

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